Many cells require additional assistance for successful microinjection. Piezo–assisted injection methods such as Piezo-ICSI can significantly increase productivity and enhance yields for a variety of micromanipulation applications. The Eppendorf PiezoXpert ensures gentle and effective penetration of the cell membrane, thereby improving the outcome of your cell manipulation experiments.

Product Information​

The Eppendorf PiezoXpert allows for piezo-assisted micromanipulation, enabling simple penetration of cells for microinjection or micromanipulation. Perforation of the cell membrane is supported by piezo impulses which are directly transferred to the capillary without any loss of intensity. Piezo-assisted micromanipulation improves the results of a variety of applications including transfer of embryonic or induced pluripotent stem cells into blastocysts and morulae, injection into mammalian oocytes and zygotes (e.g. Piezo-ICSI), and enucleation/nuclear transfer. The Eppendorf PiezoXpert forms the perfect system with Eppendorf micromanipulators, but it integrates just as easily into non-Eppendorf micromanipulation systems. The Eppendorf PiezoXpert is for research use only.

Overview: Piezo-ICSI vs. Conventional ICSI

• significantly reduced deformation of the oocyte during ICSI-related penetration
• involves a specialized Piezo-actuator to generate high-frequency, low-amplitude mechanical pulses
• use of a blunt ICSI-microcapillary to ensure optimal mechanical force transfer
• several studies have shown significantly higher oocyte survival rates

Features

1. Fast and simple sample processing due to intuitive operation
2. Reproducible parameter setting
3. Program keys to save your experimental parameters
4. Trigger impulses with an ergonomic foot control or rotary knobs right on the device
5. Optical feedback of triggered impulses
6. Additional "Clean" function for simple removal of contamination on the outside of the capillary

Applications

1. Piezo-ICSI (Intracytoplasmic Sperm Injection) in mouse, equine, porcine etc.
2. Transfer of embryonic or induced pluripotent stem cells into blastocysts or morulae
3. Enucleation/nuclear transfer
4. Blastomere biopsy from mouse embryos
5. Biopsy of equine embryos for Preimplantation Genetic Testing
6. RNA/DNA injection into fertilized oocytes of mammals